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  • 叶酸-GP C18 ( 5 µm, 120 Å, 4.6 x 250 mm )
    基本信息

    应用编号(Application ID)

    样品分类(Sample Category):医药(Pharmaceutical)

    分离模式(Separation Mode):反相(Reversed phase chromatography)

    产品系列(Product Line):GP

    方法(Method)

    色谱条件

    Column:

    GP C18 ( 5 µm, 120 Å, 4.6 x 250 mm )

    Mobile phase:

    Phosphate buffer pH 5.0 ( KH2PO4 2.0g dissolved by about 650mL water, add 15mL 0.5mol/L tetrabutylammonium hydroxide in methanol, 7mL1mol/L H3PO4 solution and 270mL methanol, cool to room temperature, adjust to pH 5.0 by NH3·H2O, then dilute to 1000mL by water )

    Flow rate:

    1.2 mL/min

    Column temperature:

    25 ℃

    Detection:

    UV 280 nm

    Injection volume:

    10 µL

    Sample: Folic Acid sample

     

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